The London Protocol was a crucial diplomatic agreement reached by the three protecting powers— the United Kingdom, France, and Russia— which aimed to resolve the ongoing disputes following the Greek War of Independence. As Greece fought for liberation from Ottoman rule, it garnered sympathy and support from these European powers, leading to their involvement in mapping out the future of Greece. In this agreement, the three powers established the borders of Greece, recognizing it as an independent state. The delineation of borders was vital in providing Greece with a sense of national identity and autonomy after prolonged foreign domination. The cities and regions included in this new territory reflected the cultural and historical significance tied to the Greek people.

The protocol stipulated that Greece would extend from the Gulf of Ambrakia to a point just below Thessaloniki, incorporating important historic regions like the Peloponnese, Attica, and parts of Central Greece. The final borders were influenced by geopolitical considerations and the desire to stabilize the region, which was marked by nationalist movements across Europe. As such, the London Protocol was not merely a territorial agreement but also a reflection of the emerging ideology of nation-states in the 19th century.

This event can be seen as a pivotal moment in the establishment of Greece as a modern nation-state, contributing to both local and regional developments in the years that followed. The recognition of Greece's borders by major European powers facilitated future diplomatic relations and influenced the balance of power in Southeast Europe. The Protocol's legacy is a source of pride for Greeks, as it symbolized the formal acknowledgment of their long struggle for independence and self-determination.