STS-101, launched on Space Shuttle Atlantis, was a vital mission aiming to resupply and assist with the construction of the International Space Station (ISS). The launch occurred from the Kennedy Space Center in Florida, lifting off at 12:29 PM EDT. Atlantis carried a crew of six astronauts, including seasoned space travelers such as Charles D. Gemar and Jeffrey S. Ashby, who each contributed their expertise to the mission's objectives.

During the mission, the crew conducted several critical tasks including the installation of new components and supplies, as well as transferring water, equipment, and other necessary materials to the ISS. One particularly notable element was the successful delivery and installation of the Integrated Cargo Carrier, which enabled the ISS to enhance its operational capabilities. The mission also provided a testbed for future flights, affirming NASA's commitment to the long-term sustainability of human presence in space.

The mission lasted for 8 days, concluding with a safe landing back on Earth. Throughout this time, Atlantis orbited the Earth multiple times, showcasing the spacecraft's capabilities in long-duration flights. STS-101 represented an important chapter in collaborative efforts among international partners, including significant contributions from the Canadian Space Agency and other partner nations invested in the ISS project.